tnbc single-cell datasets  (Broad Institute Inc)

Journal: Molecular Cancer

Article Title: NAC1 promotes stemness and regulates myeloid-derived cell status in triple-negative breast cancer

doi: 10.1186/s12943-024-02102-y

Figure Lengend Snippet: Effect of NAC1 on proliferation, migration, and invasion of TNBC cells. A Western blot of NAC1 in TNBC cells transfected si-NACC1 or si-NT. B , C Proliferation of TNBC cells with or without depletion of NAC1. D Clonogenic formation of MDA-MB-231 cells with or without depletion of NAC1. E Migration of MDA-MB-231 cells with or without depletion of NAC1. F Wound healing assay for the migratory ability of MDA-MB-231 cells with or without depletion of NAC1. G Matrigel assay for the migratory ability of MDA-MB-231 cells with or without depletion of NAC1

Article Snippet: Analysis of the TNBC single-cell Broad Institute datasets [ ] showed that NACC1 was not only expressed in tumor cells but also in various immune cells (Fig.

Techniques: Migration, Western Blot, Transfection, Wound Healing Assay, Matrigel Assay

Journal: Molecular Cancer

Article Title: NAC1 promotes stemness and regulates myeloid-derived cell status in triple-negative breast cancer

doi: 10.1186/s12943-024-02102-y

Figure Lengend Snippet: Myeloid-derived cells with expression of NAC1 supports CSCs. A Tumor growth rate for knockdown 4T1 allografted cells with or without NK cell depletion. B Expression of NAC1 in MDSCs from 4T1 tumor-bearing or tumor-free BALB/C mice. C Gr1 + /CD11b + cells were isolated from the NACC1 +/+ or NACC1 −/− mice bearing EO771 tumors through negative selection to obtain MDSCs and were further purified using CD45 positive selection assay kit (Stemcell technologies) to eliminate contaminating tumor cells. D EO771 cells co-cultured with NACC1 −/− Gr1 + /CD11b + cells showed reduced CD44 expression compared to the co-culture with NACC1 +/+ Gr1 + /CD11b + cells. E Aldolase activity of EO771 cells co-cultured with NACC1 +/+ or NACC1 −/− mice Gr1 + /CD11b + cells. F Tumor initiation ability of EO771 cells orthotopically inoculated in NACC1 +/+ or NACC1 −/− mice ( n = 5, Tn = 2). G-I EO771 cells were co-cultured with NACC1 +/+ or NACC1 −/− Gr1 + /CD11b + cells, and cell viability was determined using the CellTrace™ CFSE Cell Proliferation Kit ( G ) or luciferase assay ( H )

Article Snippet: Analysis of the TNBC single-cell Broad Institute datasets [ ] showed that NACC1 was not only expressed in tumor cells but also in various immune cells (Fig.

Techniques: Derivative Assay, Expressing, Knockdown, Isolation, Selection, Purification, Cell Culture, Co-Culture Assay, Activity Assay, Luciferase

Journal: Cancers

Article Title: High PANX1 Expression Leads to Neutrophil Recruitment and the Formation of a High Adenosine Immunosuppressive Tumor Microenvironment in Basal-like Breast Cancer

doi: 10.3390/cancers14143369

Figure Lengend Snippet: Clinical baseline characteristics of the included patients and corresponding experimental procedures for the specimens.

Article Snippet: The TNBC single-cell dataset [ ] was downloaded from the Broad Institute Single Cell Portal ( https://singlecell.broadinstitute.org/single_cell/study/SCP1106/ ) (accessed on 7 September 2021).

Techniques: Immunohistochemistry-IF

Journal: Cancers

Article Title: High PANX1 Expression Leads to Neutrophil Recruitment and the Formation of a High Adenosine Immunosuppressive Tumor Microenvironment in Basal-like Breast Cancer

doi: 10.3390/cancers14143369

Figure Lengend Snippet: Clinical characteristics of the included samples for immunohistochemistry.

Article Snippet: The TNBC single-cell dataset [ ] was downloaded from the Broad Institute Single Cell Portal ( https://singlecell.broadinstitute.org/single_cell/study/SCP1106/ ) (accessed on 7 September 2021).

Techniques: Immunohistochemistry